Peptide ELISA: Antibody detection limit dilution 1/16000. Western blot: 1-3 μg/mL. Approx.48 kDa band observed in Human Brain, Mouse Brainlysates and in lysates of Mouse putuitary cell line NIH3T3 (calculated MW of 51.8 kDaaccording to NP_004227.1). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Thyroid Gland shows nclear and cytoplasm staining inactivated epithelial cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
TRIP15 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP. TRIP15 is also involved in early stage of neuronal differentiation via its interaction with NIF3L1.Synonyms: Alien homolog, COP9 signalosome complex subunit 2, CSN2, JAB1-containing signalosome subunit 2, SGN2, TRIP-15, Thyroid receptor-interacting protein 15