Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
序列
C-KRKRETDDEG EDD
特异性
This antibody does NOT recognize the TNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).
交叉反应 (详细)
Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow. Species reactivity (tested):Human.
纯化方法
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
免疫原
Peptide from C Terminus of the protein sequence according to NP_006392.1 Genename: ANP32B
Peptide ELISA: Limit dilution: 1/1000. Western blot: 0.3-1 μg/mL. Approx 26 kDa band observed in Human lymph node lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
APRIL antibody, PHAPI2 antibody, SSP29 antibody, 2410015B15Rik antibody, PAL31 antibody, PHAPI2a antibody, Ssp29 antibody, cb908 antibody, fa99a11 antibody, wu:fa28c10 antibody, wu:fa99a11 antibody, wu:fb40e12 antibody, zgc:77745 antibody, hLAMP-1 antibody, MGC80871 antibody, ANP32B antibody, MGC69373 antibody, SLAN antibody, acidic nuclear phosphoprotein 32 family member B antibody, acidic (leucine-rich) nuclear phosphoprotein 32 family, member B antibody, acidic nuclear phosphoprotein 32 family member B L homeolog antibody, acidic leucine-rich nuclear phosphoprotein 32 family member B antibody, ANP32B antibody, Anp32b antibody, anp32b antibody, anp32b.L antibody, LOC494442 antibody
背景
PHAPI2 / APRIL is a multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. It is required for the progression from the G1 to the S phase. It is an anti-apoptotic protein which functions as a caspase-3 inhibitor. It has no phosphatase 2A (PP2A) inhibitor activity.Synonyms: APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, PHAPI2, SSP29, Silver-stainable protein SSP29