Peptide ELISA: Limit dilution 1: 16000. Western blot: Experiments gave an approx 60 kDa band in Human Liver and Brain lysates at1 μg/mL. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Placenta shows strongstaining of cytoplasm in syncytiotrophoblasts. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Human NEIL1 is the homologs of Escherichia coli Nei. Human NEIL1 is a DNA glycosylase, that phylogenetically belongs to the Fpg/Nei family, primarily found in the bacterial kingdom and is functionally homologous to bacterial Nei. Thymine glycol, a potentially lethal DNA lesion produced by reactive oxygen species, can be removed by DNA glycosylases. The NEIL1 gene is located on the long arm of chromosome 15 that is frequently deleted in human cancers.Synonyms: DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, NEH1