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KAP1 抗体 (C-Term)

Name: KAP1 抗体 (C-Term)
SKU: ABIN374296
Availability: OutOfStock

TRIM28 适用: 人, 小鼠 WB, IF, IHC (p), ChIP, EIA 宿主: 山羊 Polyclonal unconjugated

Image no. 2 for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN374296) (KAP1 抗体 (C-Term))

Image no. 1 for anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody (ABIN374296) (KAP1 抗体 (C-Term))

2 images

(2 references)

产品编号 ABIN374296

发货至: 中国

Datasheet as PDF

抗原 See all KAP1 (TRIM28) 抗体

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
抗原表位
22

15

9

8

6

6

5

5

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1
C-Term
适用
101

45

41

10

8

8

7

5

4

2

1

1

1
人, 小鼠
宿主
103

14

4
山羊
Compatible Secondaries
克隆类型
98

23
多克隆
标记
61

8

8

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This KAP1 antibody is un-conjugated
应用范围
80

37

36

27

26

22

22

21

19

13

6

4

4

3

2

2

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

序列

C-SSQELSGGPG DGP

特异性

This antibody detects TIF1-beta / TRIM28 at C-term.

交叉反应（详细）

Species reactivity (tested):Human and Mouse.

纯化方法

Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

免疫原

Synthetic peptide from the C-Terminus sequence according to NP_005753.1 Genename: TRIM28

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anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibody
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TRIM28 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated

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TRIM28 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (pSer824) antibody
TRIM28 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.2-1 μg/mL. Approx 100-120 kDa band seen in HepG2 lysate. Immunohistochemistry: 5 μg/mL (Ssuccessfully used on Human gastric cancer surgical

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Santanelli, Paolini, Longo: "Comments on: Improving breast cancer surgery: a classification and quadrant per quadrant atlas for oncoplastic surgery (Clough KB, Kaufman GJ, et al. ASO DOI 10.1245/s10434-009-0792-y)." in: Annals of surgical oncology, Vol. 18 Suppl 3, pp. S257-8; author reply S259-60, (2011) (PubMed).

Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).
抗原

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

别名

TIF1-beta / TRIM28 (TRIM28 产品)

背景

The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1). KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.Synonyms: KAP-1, KAP1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, KRIP1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TIF1 beta, TIF1B, Transcription intermediary factor 1-beta, Tripartite motif-containing protein 28

基因ID

10155

NCBI登录号

NP_005753

UniProt

Q13263

途径

Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus