Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-0.5 μg/mL. Approx 30-35 kDa band seen in Human Liver and Kidneylysates [Calculated MW of 30.5 kDa according to NP_001748.1]. Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Kidney showscytoplasmic staining of epithelial cells of renal tubules. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Carbonyl reductase is one of several monomeric, NADPH dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q.Synonyms: 15-hydroxyprostaglandin dehydrogenase [NADP+], CBR, CBR1, CRN, NADPH-dependent carbonyl reductase 1, Prostaglandin 9-ketoreductase, Prostaglandin-E(2) 9-reductase