Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.1-0.3 μg/mL. Experiments gave strong bands at 75-90 kDa and a faint50- kDa band in Human Duodenal lysate after 0.1 μg/mL antibody staining. These bandscorrespond to earlier findings in literature (Piccinni et al, J. Biol. Chem. , Vol.279 (10),9103-9114). All these bands were successfully blocked by incubation with the immunizingpeptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.Synonyms: EZF, Epithelial zinc finger protein EZF, GKL, Gut-enriched krueppel-like factor, Krueppel-like factor 4