This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
免疫原
Peptide from C Terminus of the protein sequence according to NP_387506.1, NP_004623.1 Genename: DAP3
Peptide ELISA: 1/16000. Western blot: 1 - 3 μg/mL. Approx 40-45 kDa band seen in HeLa lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.Synonyms: 28S ribosomal protein S29 mitochondrial, DAP-3, Death-associated protein 3, Ionizing radiation resistance conferring protein, MRP-S29