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ELMO1 抗体 (C-Term)

Name: ELMO1 抗体 (C-Term)
SKU: ABIN374159
Availability: OutOfStock

ELMO1 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 犬 WB, IHC (p), EIA 宿主: 山羊 Polyclonal unconjugated

Image no. 1 for anti-Engulfment and Cell Motility 1 (ELMO1) (C-Term) antibody (ABIN374159) (ELMO1 抗体 (C-Term))

1 image

(4 references)

产品编号 ABIN374159

发货至: 中国

Datasheet as PDF

抗原 See all ELMO1 抗体

ELMO1 (Engulfment and Cell Motility 1 (ELMO1))
抗原表位
15

6

4

3

3

2

1

1

1

1
C-Term
适用
55

24

14

2

2

2

2

2

2

1

1

1

1

1

1
人, 小鼠, 大鼠, Cow, 斑马鱼, 犬
宿主
52

2

1
山羊
Compatible Secondaries
克隆类型
46

9
多克隆
标记
19

5

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This ELMO1 antibody is un-conjugated
应用范围
37

18

13

13

5

3

3

3

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

序列

PKEPSNYDFV YDCN

特异性

This antibody is expected to recognise both Human isoforms of this protein (NP_569709.1 and NP_001034548.1 represent the same isoform).

交叉反应（详细）

Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human, Bovine, Zebrafish.

纯化方法

Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

免疫原

Peptide from C-Terminus of the protein sequence according to NP_055615.8, NP_569709.1, NP_001034548.1 Genename: ELMO1

Top Product

Discover our top product ELMO1 Primary Antibody
anti-Engulfment and Cell Motility 1 (ELMO1) (C-Term) antibody
ELMO1 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) (C-Term) antibody
Verified ELMO1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) antibody
ELMO1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) (AA 372-421) antibody
ELMO1 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 犬, 马, 兔, 豚鼠, Bat, 小鸡, Hamster, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) (N-Term) antibody
ELMO1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) (AA 520-727) antibody
ELMO1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) antibody
ELMO1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) (N-Term) antibody
ELMO1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) (AA 1-727) antibody
ELMO1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Engulfment and Cell Motility 1 (ELMO1) (AA 527-727) antibody
ELMO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Peptide ELISA: Limit dilution 1: 32000. Western blot: Recommended for use at 1-2 μg/mL. Approx 70 kDa and 50 kDa bands seen inJurkat cell lysate. Note the smaller isoform of this protein has a consensus glycosylationsite, which may explain the higher than expected band size (50 kDa versus 30 kDa). Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B. D and Rickwood, D. ,eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Epting, Slanchev, Boehlke, Hoff, Loges, Yasunaga, Indorf, Nestel, Lienkamp, Omran, Kuehn, Ronneberger, Walz, Kramer-Zucker: "The Rac1 regulator ELMO controls basal body migration and docking in multiciliated cells through interaction with Ezrin." in: Development (Cambridge, England), Vol. 142, Issue 1, pp. 174-84, (2014) (PubMed).

Petrosyan, Ali, Verma, Cheng, Cheng: "Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 7, pp. 1153-65, (2012) (PubMed).

Huenniger, Krämer, Soom, Chang, Köhler, Depping, Kehlenbach, Kaether: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).

Vassilieva, Gerner-Smidt, Ivanov, Nusrat: "Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G965-76, (2008) (PubMed).
抗原

ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

别名

ELMO1 (ELMO1 产品)

背景

ELMO1 is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. ELMO1 acts in association with DOCK1 and CRK and was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. It is belived to enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. ELMO1 interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. It is part of a complex with DOCK1 and RAC1 and with DOCK1 and CRK isoform CRK-II. Recent studies show that it interacts with PLEKHG6.Synonyms: CED-12 homolog, ELMO 1, ELMO-1, Engulfment and cell motility protein 1, KIAA0281

基因ID

9844

NCBI登录号

NP_055615

UniProt

Q92556