NUMB 抗体 (C-Term)
(1 reference)-
- 抗原 See all NUMB 抗体
- NUMB (Numb Homolog (NUMB))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 犬, Cow
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This NUMB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 序列
- C-PFSSDLQKTF EIEL
- 特异性
- This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
- 交叉反应 （详细）
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Species reactivity (expected):Canine (Dog) and Bovine.
Species reactivity (tested):Human, Mouse. - 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity chromatography using the immunizing peptide
- 免疫原
- Synthetic peptide from the C-Terminus of Human NUMB (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1) Genename: NUMB
- Top Product
- Discover our top product NUMB Primary Antibody
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anti-Numb Homolog (NUMB) (C-Term) antibody
Verified NUMB 适用: 人, 小鼠 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 521-640) antibodyNUMB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-135) antibodyNUMB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A7-A6 unconjugated
anti-Numb Homolog (NUMB) (AA 1-50) antibodyNUMB 适用: 人, 小鼠, 大鼠, Cow, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (pSer276) antibodyNUMB 适用: 小鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-135) antibodyNUMB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (C-Term) antibodyNUMB 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-214) antibodyNUMB 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 376-482) antibodyNUMB 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 377-642) antibodyNUMB 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.03-0.1 μg/mL. Approx 80 kDa band observed in Mouse Brain lysates(calculated MW of 70.0 kDa according to NP_001005743.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "The clathrin adaptor Numb regulates intestinal cholesterol absorption through dynamic interaction with NPC1L1." in: Nature medicine, Vol. 20, Issue 1, pp. 80-6, (2014) (PubMed).
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: "The clathrin adaptor Numb regulates intestinal cholesterol absorption through dynamic interaction with NPC1L1." in: Nature medicine, Vol. 20, Issue 1, pp. 80-6, (2014) (PubMed).
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- 抗原
- NUMB (Numb Homolog (NUMB))
- 别名
- NUMB (NUMB 产品)
- 背景
- Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelatorSynonyms: Protein S171, Protein numb homolog, h-Numb
- 基因ID
- 8650
- NCBI登录号
- NP_001005743
- UniProt
- P49757
- 途径
- Cell-Cell Junction Organization
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