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Cortactin 抗体 (N-Term)

Name: Cortactin 抗体 (N-Term)
SKU: ABIN374140
Availability: OutOfStock

CTTN 适用: 人, 小鼠, 大鼠, 犬, Cow WB 宿主: 山羊 Polyclonal unconjugated

Image no. 1 for anti-Cortactin (CTTN) (N-Term) antibody (ABIN374140) (Cortactin 抗体 (N-Term))

1 image

产品编号 ABIN374140

发货至: 中国

Datasheet as PDF

抗原 See all Cortactin (CTTN) 抗体

Cortactin (CTTN)
抗原表位
15

13

12

7

7

6

6

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
121

60

43

5

4

2

2

2

2

2

2

1

1

1
人, 小鼠, 大鼠, 犬, Cow
宿主
120

7

1
山羊
Compatible Secondaries
克隆类型
111

17
多克隆
标记
76

7

4

4

4

4

4

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
This Cortactin antibody is un-conjugated
应用范围
93

50

48

16

15

14

14

12

11

6

3

3

1

1

1

1
Western Blotting (WB)

序列

WKASAGHAVS IA-C

特异性

This antibody detects Cortactin at N-term.

纯化方法

Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

免疫原

Peptide from N Terminus of the protein sequence according to NP_005222.2, NP_612632.1 Genename: CTTN

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CTTN 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

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CTTN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

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CTTN 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6 unconjugated

anti-Cortactin (CTTN) (AA 1-509) antibody
CTTN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cortactin (CTTN) (AA 1-509) antibody
CTTN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cortactin (CTTN) (pTyr421) antibody
CTTN 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cortactin (CTTN) (N-Term) antibody
Verified CTTN 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

应用备注

Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.01-0.03 μg/mL. Approx.80 kDa band observed in Mouse and Rat Brainlysates (calculated MW of 61.0 kDa according to Human NP_005222.2 and of 61.0 kDaaccording to Mouse NP_031829.2). The observed molecular weight corresponds to earlierfindings in literature with different antibodies (Paradis et al, J Cell Sci.2008 Jun 15,121(Pt12): 1965-72, PMID 18495841).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
抗原

Cortactin (CTTN)

别名

Cortactin (CTTN 产品)

背景

Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1

基因ID

2017

NCBI登录号

NP_005222

UniProt

Q14247

途径

MAPK Pathway