PRAM1
适用: 人
IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 350
应用备注
Peptide ELISA: Limit dilution 1: 8000. Western blot: 1-3 μg/mL (overnight incubation). Approx 90-100 kDa band seen in Jurkat celllysates. Please note that although the predicted MW of PRAM-1 is 70 kDa, Moog-Lutz et al. ,(J. Biol. Chem, Jun 2001, 276: 22375 - 22381) detected aPRAM-1 migrating with an apparentmolecular mass of 97 kDa and this is what we observed as well. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumi
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.