MAPK14 抗体 (pThr180, pTyr182)
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- 抗原 See all MAPK14 抗体
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
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抗原表位
- pThr180, pTyr182
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适用
- 人, 小鼠, 大鼠, 犬, 小鸡, Cow, 斑马鱼, Non-Human Primate
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MAPK14 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognizes the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
- 交叉反应 (详细)
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Species reactivity (expected):Bovine, Canine, Chicken, Mouse, Zebrafish and non-Human Primates.
Species reactivity (tested):Human. - 纯化方法
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- 免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr180 and phospho-Tyr182 of rat p38 MAPK.
- 亚型
- IgG
- Top Product
- Discover our top product MAPK14 Primary Antibody
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- 应用备注
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Western blot: 1/1000. Immunohistochemistry: 1/250-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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Methyl gallate isolated from Spondias pinnata exhibits anticancer activity against human glioblastoma by induction of apoptosis and sustained extracellular signal-regulated kinase 1/2 activation." in: Pharmacognosy magazine, Vol. 11, Issue 42, pp. 269-76, (2015) (PubMed).
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Methyl gallate isolated from Spondias pinnata exhibits anticancer activity against human glioblastoma by induction of apoptosis and sustained extracellular signal-regulated kinase 1/2 activation." in: Pharmacognosy magazine, Vol. 11, Issue 42, pp. 269-76, (2015) (PubMed).
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- 抗原
- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
- 别名
- MAP Kinase p38 alpha / MAPK14 (MAPK14 产品)
- 背景
- The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002, Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003, Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182 (Lin et al., 1995).Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha
- 基因ID
- 1432
- NCBI登录号
- NP_001306
- UniProt
- Q16539
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins
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