Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)
特异性
This antibody recognizes the ~48k alpha-1 and alpha-2 Subunits of the Glycine Receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other Glycine Receptor subunits.
交叉反应 (详细)
Species reactivity (tested):Human, Mouse and Rat.
纯化方法
Affinity Chromatography.
免疫原
Peptide from the N-terminus region of the alpha-1 subunit of rat Glycine Receptor.
Dot blot: 1/1000. Western blot: 1/1000. Immunohistochemistry: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in 100 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
缓冲液
5 mM Ammonium Bicarbonate.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
After reconstitution: Store the antibody undiluted (in aliquots) at-20 °C.
Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001, Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).Synonyms: GLRA1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit