(11 references)-
- 抗原 See all CD28 抗体
- CD28
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适用
- 小鼠
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宿主
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仓鼠
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克隆类型
- 单克隆
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标记
- This CD28 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)
- 产品特性
- Synonyms: TP44, T-cell-specific surface glycoprotein CD28
- 纯化方法
- Purified
- 克隆位点
- 37-51
- 亚型
- IgG
- Top Product
- Discover our top product CD28 Primary Antibody
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) (AA 19-152) antibodyCD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Flow Cytometry. (Ref.2-5)Immunoprecipitation. (Ref.2)Functional Assay: In vitro costimulation of T cells and NK cells. (Ref.1,4,7)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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: "Myeloid expression of angiotensin-converting enzyme facilitates myeloid maturation and inhibits the development of myeloid-derived suppressor cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 94, Issue 5, pp. 536-44, (2014) (PubMed).
: "Severe thymic atrophy in a mouse model of skin inflammation accounts for impaired TNFR1 signaling." in: PLoS ONE, Vol. 7, Issue 10, pp. e47321, (2012) (PubMed).
: "Signal integration by Akt regulates CD8 T cell effector and memory differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 9, pp. 4305-14, (2012) (PubMed).
: "CD8+ T-cells expressing interferon gamma or perforin play antagonistic roles in heart injury in experimental Trypanosoma cruzi-elicited cardiomyopathy." in: PLoS pathogens, Vol. 8, Issue 4, pp. e1002645, (2012) (PubMed).
: "Selective CD28 blockade attenuates acute and chronic rejection of murine cardiac allografts in a CTLA-4-dependent manner." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 11, Issue 8, pp. 1599-609, (2011) (PubMed).
: "KSR1 modulates the sensitivity of mitogen-activated protein kinase pathway activation in T cells without altering fundamental system outputs." in: Molecular and cellular biology, Vol. 29, Issue 8, pp. 2082-91, (2009) (PubMed).
: "Bcl6 controls granzyme B expression in effector CD8+ T cells." in: European journal of immunology, Vol. 36, Issue 12, pp. 3146-56, (2006) (PubMed).
: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).
: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, (1996) (PubMed).
: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, (1994) (PubMed).
: "Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 125, Issue 6, pp. 2473-7, (1981) (PubMed).
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: "Myeloid expression of angiotensin-converting enzyme facilitates myeloid maturation and inhibits the development of myeloid-derived suppressor cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 94, Issue 5, pp. 536-44, (2014) (PubMed).
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- 抗原
- CD28
- 别名
- CD28 (CD28 产品)
- 背景
- CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. (Ref.1-4) CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses. (Ref.5-8)Synonyms: T-cell-specific surface glycoprotein CD28, TP44
- UniProt
- P31041
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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