Nestin 抗体
-
- 抗原 See all Nestin (NES) 抗体
- Nestin (NES)
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Nestin antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human Nestin with no cross- reactivity to rodent
- 纯化方法
- Protein G purified
- 免疫原
- A 150-amino-acid fragment from the cloned human Nestin conjugated to GST.
- 克隆位点
- 2C1-3A11
- 亚型
- IgG1
- Top Product
- Discover our top product NES Primary Antibody
-
-
- 应用备注
-
Approved: Flo, ICC (1:100 - 1:500), IHC, IHC-P (20 μg/mL), IP, WB (1:1000 - 1:5000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL. - 说明
-
Target Species of Antibody: Human
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-