HIF1A 抗体 (AA 432-528)
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- 抗原 See all HIF1A 抗体
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
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抗原表位
- AA 432-528
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适用
- 人, 小鼠, 大鼠, Cow, Pig, 猴, 绵羊, 雪貂
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HIF1A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)
- 特异性
- Reacts with human HIF-1 alpha
- 交叉反应
- 小鼠, 牛, 兔, 绵羊
- 交叉反应 (详细)
- Cross reacts with proteins from monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret.
- 纯化方法
- Purified (protein A)
- 免疫原
- Fusion protein containing amino acids 432-528 of human HIF-1alpha
- 克隆位点
- H1alpha67
- 亚型
- IgG2b
- Top Product
- Discover our top product HIF1A Primary Antibody
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- 应用备注
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Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC1:100-1:300 WB1:500-1:1000 ICC/ IF1:100-1:500 IP1:10 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.5 mg/mL
- 缓冲液
- Tris-glycine, 150 mM NaCl pH 7.5, Sodium azide 0.05 %
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 4°C. Do not freeze.
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- 抗原
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- 别名
- Hypoxia-Inducible Factor 1-alpha (HIF1A 产品)
- 别名
- hif1a antibody, AA959795 antibody, HIF1alpha antibody, MOP1 antibody, bHLHe78 antibody, HIF-1A antibody, HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, PASD8 antibody, HIF1-alpha antibody, hif-1a antibody, hypoxia inducible factor 1 alpha antibody, hypoxia inducible factor 1, alpha subunit antibody, hypoxia inducible factor 1 alpha subunit antibody, HIF (Hypoxia Inducible Factor) homologa antibody, hif-1a antibody, Hif1a antibody, HIF1A antibody, hif1a antibody, hif-1 antibody
- 背景
- HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes.
- 基因ID
- 3091
- UniProt
- Q16665
- 途径
- Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect
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