(1 reference)-
- 抗原
- SAPK, JNK
-
抗原表位
- AA 181-185
- 适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
-
应用范围
- Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- The antibody detects endogenous level of total SAPK/JNK protein.
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 免疫原
- Peptide sequence around AA 181-185 (M-M-T-P-Y) derived from Human SAPK, JNK. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
- 亚型
- IgG
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anti-SAPK, JNK (pTyr185) antibody
适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SAPK, JNK (pThr183) antibody适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SAPK, JNK (AA 166-215) antibody适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-SAPK, JNK (AA 151-200), (pTyr185) antibody适用: 人, 小鼠, 大鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
-
Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
-
-
: "Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts." in: Protein expression and purification, Vol. 75, Issue 2, pp. 138-46, (2010) (PubMed).
-
: "Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts." in: Protein expression and purification, Vol. 75, Issue 2, pp. 138-46, (2010) (PubMed).
-
- 抗原
- SAPK, JNK
- 别名
- SAPK/JNK
- 背景
- Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
- 分子量
- 54 kDa
- NCBI登录号
- NP_001128
- UniProt
- P53779
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