The antibody detects endogenous level of taotal Rb protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
免疫原
Peptide sequence around AA 778-782 (T-L-S-P-I) derived from Human Rb. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Braumüller, Wieder, Brenner, Aßmann, Hahn, Alkhaled, Schilbach, Essmann, Kneilling, Griessinger, Ranta, Ullrich, Mocikat, Braungart, Mehra, Fehrenbacher, Berdel, Niessner, Meier, van den Broek et al.: "T-helper-1-cell cytokines drive cancer into senescence. ..." in: Nature, Vol. 494, Issue 7437, pp. 361-5, (2013) (PubMed).
Cai, Ye, Liu, Lu, Lu, Zhang, Wang, Cao: "Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 25, Issue 7, pp. 1385-91, (2011) (PubMed).
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.