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- 抗原 See all ASK1 (MAP3K5) 抗体
- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
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抗原表位
- pSer83
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ASK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- The antibody detects endogenous level of ASK1 only when phosphorylated at serine 83.
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- Peptide sequence around phosphorylation site of pSer83 (G-S-S (p) -V-G) derived from Human ASK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- 亚型
- IgG
- Top Product
- Discover our top product MAP3K5 Primary Antibody
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- 应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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Targeting p53 via JNK pathway: a novel role of RITA for apoptotic signaling in multiple myeloma." in: PLoS ONE, Vol. 7, Issue 1, pp. e30215, (2012) (PubMed).
: "ASK1-JNK signaling cascade mediates Ad-ST13-induced apoptosis in colorectal HCT116 cells." in: Journal of cellular biochemistry, Vol. 110, Issue 3, pp. 581-8, (2010) (PubMed).
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Targeting p53 via JNK pathway: a novel role of RITA for apoptotic signaling in multiple myeloma." in: PLoS ONE, Vol. 7, Issue 1, pp. e30215, (2012) (PubMed).
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- 抗原
- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
- 别名
- ASK1 (MAP3K5 产品)
- 别名
- ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody
- 背景
- Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively.
- 分子量
- 155 kDa
- NCBI登录号
- NP_005914
- UniProt
- Q99683
- 途径
- MAPK Pathway, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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