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- 抗原 See all CDC25A 抗体
- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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抗原表位
- pSer76
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CDC25A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- The antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- Peptide sequence around phosphorylation site of pSer76 (M-G-S (p) -S-E) derived from Human cdc25A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- 亚型
- IgG
- Top Product
- Discover our top product CDC25A Primary Antibody
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- 应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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CK1ε targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 3, pp. 531-7, (2011) (PubMed).
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CK1ε targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 3, pp. 531-7, (2011) (PubMed).
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- 抗原
- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
- 别名
- cdc25A (CDC25A 产品)
- 别名
- cdc25a2 antibody, cdc25A antibody, sb:cb317 antibody, CDC25A2 antibody, D9Ertd393e antibody, cell division cycle 25A antibody, cell division cycle 25B antibody, cell division cycle 25A S homeolog antibody, CDC25A antibody, cdc25a antibody, cdc25b antibody, Cdc25a antibody, cdc25a.S antibody
- 背景
- Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
- 分子量
- 70 kDa
- NCBI登录号
- NP_001780
- UniProt
- P30304
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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