Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
The antibody detects endogenous level of FKHR only when phosphorylated at serine 319.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pSer319 (T-S-S (p) -N-A) derived from Human FKHR, FOXO1A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14