Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
The antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pThr394 (P-G-T (p) -P-T) derived from Human MEK-2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.