Rab4 抗体 (C-Term)
(1 reference)-
- 抗原 See all Rab4 (RAB4A) 抗体
- Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Rab4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~26 kDa.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Peptide Affinity Purified
- 免疫原
- C-terminal peptide from human Rab4
- Top Product
- Discover our top product RAB4A Primary Antibody
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anti-RAB4A, Member RAS Oncogene Family (RAB4A) antibody
RAB4A 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 4-00E-11 unconjugated
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (AA 1-218) antibodyRAB4A 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1C10 unconjugated
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (HRP)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (FITC)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (Alkaline Phosphatase (AP))RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (Biotin)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (PE)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal PE
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (APC)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal APC
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (PerCP)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal PerCP
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (Atto 488)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Atto 488
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- 应用备注
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- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:150)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN361840 was sufficient for detection of Rab4 in 10 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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: "Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output." in: Blood, Vol. 118, Issue 3, pp. 816-26, (2011) (PubMed).
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: "Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output." in: Blood, Vol. 118, Issue 3, pp. 816-26, (2011) (PubMed).
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- 抗原
- Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))
- 别名
- Rab4 (RAB4A 产品)
- 别名
- rab4a antibody, MGC52945 antibody, fc93b03 antibody, wu:fc93b03 antibody, RAB4A antibody, HRES-1/RAB4 antibody, RAB4 antibody, AI848268 antibody, Rab4 antibody, RAB4A, member RAS oncogene family antibody, RAB4a, member RAS oncogene family antibody, rab4a antibody, RAB4A antibody, Rab4a antibody
- 背景
- Rab4 is a 25 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4). In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).
- 基因ID
- 5867
- NCBI登录号
- NP_004569
- UniProt
- P20338
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