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Calreticulin 抗体

Name: Calreticulin 抗体
Brand: antibodies-online
SKU: ABIN361835
Availability: OutOfStock

CALR 适用: 人 WB, IHC, IP, FACS, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of multiple cell lines lysates showing detection of Calreticulin protein using Rabbit Anti-Calreticulin Polyclonal Antibody (ABIN361834 and ABIN361835). (Calreticulin 抗体)

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calreticulin Polyclonal Antibody (ABIN361834 and ABIN361835). (Calreticulin 抗体)

ICC

Calreticulin, Mouse backskin. (Calreticulin 抗体)

ICC

4 images

(6 references)

产品编号 ABIN361835

发货至: 中国

Datasheet as PDF

抗原 See all Calreticulin (CALR) 抗体

Calreticulin (CALR)
适用
129

69

52

10

9

7

7

6

5

4

4

4

3

1

1

1

1

1
人
宿主
123

9

2

1
兔
Compatible Secondaries
克隆类型
118

17
多克隆
标记
62

13

11

7

5

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Calreticulin antibody is un-conjugated
应用范围
99

51

46

35

25

22

21

16

14

13

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Detects ~63 kDa.

交叉反应

小鸡, Cow, 犬, 豚鼠, Hamster, 人, 猴, 小鼠, Pig, 兔, 大鼠, 绵羊

纯化方法

Peptide Affinity Purified

免疫原

Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH

Top Product

Discover our top product CALR Primary Antibody
anti-Calreticulin (CALR) (AA 196-310) antibody
CALR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calreticulin (CALR) (AA 18-193) antibody
CALR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calreticulin (CALR) (AA 18-417) antibody
CALR 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7B3D7 unconjugated

anti-Calreticulin (CALR) antibody
CALR 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 1G6A7 unconjugated

anti-Calreticulin (CALR) (N-Term) antibody
CALR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calreticulin (CALR) (AA 1-417) antibody
CALR 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1G11-1A9 unconjugated

anti-Calreticulin (CALR) (AA 277-305) antibody
CALR 适用: 人, 大鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21112 unconjugated

anti-Calreticulin (CALR) antibody (HRP)
CALR 适用: 人 WB, IHC, IP, FACS, IF, ICC 宿主: 兔 Polyclonal HRP

anti-Calreticulin (CALR) antibody (FITC)
CALR 适用: 人 WB, IHC, IP, FACS, IF, ICC 宿主: 兔 Polyclonal FITC

anti-Calreticulin (CALR) antibody (Biotin)
CALR 适用: 人 WB, IHC, IP, FACS, IF, ICC 宿主: 兔 Polyclonal Biotin

应用备注
- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
说明

A 1:1000 dilution of ABIN361834 was sufficient for detection of Calreticulin in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
Schultheis, Smith, Kiosses, Kraynyak, Wong, Oh, Broderick: "Delineating the Cellular Mechanisms Associated with Skin Electroporation." in: Human gene therapy methods, Vol. 29, Issue 4, pp. 177-188, (2019) (PubMed).

Koide, Kondo, Odkhuu, Ulziisaikhan, Ukaji, Yokochi, Umezawa: "Inhibition of receptor activator of nuclear factor-κB ligand- or lipopolysaccharide-induced osteoclast formation by conophylline through downregulation of CREB." in: Immunology letters, Vol. 161, Issue 1, pp. 31-7, (2014) (PubMed).

Kobayashi, Hoshinaga, Miura, Tokuda, Shigeoka, Murai, Horio: "Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats." in: Bioscience, biotechnology, and biochemistry, Vol. 78, Issue 6, pp. 1060-6, (2014) (PubMed).

Frenz, Graalmann, Detje, Döring, Grabski, Scheu, Kalinke et al.: "Independent of plasmacytoid dendritic cell (pDC) infection, pDC triggered by virus-infected cells mount enhanced type I IFN responses of different composition as opposed to pDC stimulated with free ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 5, pp. 2496-503, (2014) (PubMed).

Kluge, Mack, Iyer, Pujol, Heigele, Learn, Usmani, Sauter, Joas, Hotter, Bibollet-Ruche, Plenderleith, Peeters, Geyer, Sharp, Fackler, Hahn, Kirchhoff: "Nef proteins of epidemic HIV-1 group O strains antagonize human tetherin." in: Cell host & microbe, Vol. 16, Issue 5, pp. 639-50, (2014) (PubMed).

Tongu, Harashima, Yamada, Harada, Harada: "Immunogenic chemotherapy with cyclophosphamide and doxorubicin against established murine carcinoma." in: Cancer immunology, immunotherapy : CII, Vol. 59, Issue 5, pp. 769-77, (2010) (PubMed).
抗原

Calreticulin (CALR)

别名

Calreticulin (CALR 产品)

别名

CRT antibody, RO antibody, SSA antibody, cC1qR antibody, crc antibody, crt antibody, Calregulin antibody, CG9429 antibody, Clr antibody, Dmel\\CG9429 antibody, anon-EST:Liang-2.11 antibody, anon-EST:Liang-2.17 antibody, anon-EST:fe3D7 antibody, clone 2.11 antibody, clone 2.17 antibody, eth antibody, potp antibody, cc1qr antibody, ssa antibody, NV19007 antibody, CRP55 antibody, ERp60 antibody, HACBP antibody, CHOT1 antibody, CHT1 antibody, CT1 antibody, AA589632 antibody, CRTR antibody, Creat antibody, CCDS1 antibody, calrl2 antibody, fc66d10 antibody, wu:fc66d10 antibody, wu:fj50c03 antibody, zgc:66153 antibody, calreticulin antibody, calreticulin L homeolog antibody, Calreticulin antibody, putative calreticulin antibody, solute carrier family 6 member 8 antibody, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 antibody, CALR antibody, calr.L antibody, Crt antibody, crt antibody, CRT antibody, Calr antibody, calr antibody, LMJF_31_2600 antibody, Crc antibody, Slc6a8 antibody, SLC6A8 antibody, LOC8269812 antibody

背景

Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).

基因ID

811

NCBI登录号

NP_004334

UniProt

P27797

途径

Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response