Lysine (lys) (acetylated) 抗体
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- 抗原
- Lysine (lys)
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抗原表位
- acetylated
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)
- 特异性
- Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.
- 纯化方法
- Protein G Purified
- 免疫原
- Acetylated KLH
- 克隆位点
- 7F8
- 亚型
- IgG1
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- 应用备注
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- WB (1:1000)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg of ABIN361802 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 μg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- Lysine (lys)
- 别名
- Lysine
- 别名
- lysine antibody, lys antibody
- 物质类
- Amino Acid
- 背景
- Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).
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