我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

TLR4 抗体 (AA 420-435)

Name: TLR4 抗体 (AA 420-435)
Brand: antibodies-online
SKU: ABIN361724
Availability: OutOfStock

TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

TLR4 MD 2 recomb, 95kD. (TLR4 抗体 (AA 420-435))

Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (ABIN361724). (TLR4 抗体 (AA 420-435))

IHC

Image no. 2 for anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (ABIN361724) (TLR4 抗体 (AA 420-435))

Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (ABIN361724). (TLR4 抗体 (AA 420-435))

IHC

Flow Cytometry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (ABIN361724). (TLR4 抗体 (AA 420-435))

FACS

6 images

(3 references)

产品编号 ABIN361724

发货至: 中国

Datasheet as PDF

抗原 See all TLR4 抗体

TLR4 (Toll-Like Receptor 4 (TLR4))
抗原表位
16

16

16

15

8

7

7

5

4

4

4

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 420-435
适用
178

90

72

37

23

20

19

19

5

2

1

1
人
宿主
129

71

9

4
兔
Compatible Secondaries
克隆类型
133

80
多克隆
标记
90

20

20

16

7

6

3

3

3

3

3

3

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TLR4 antibody is un-conjugated
应用范围
147

128

49

45

32

28

27

26

26

23

14

8

8

7

5

5

4

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Detects ~75-80 kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

交叉反应

人, 小鼠

纯化方法

Protein A Purified

免疫原

Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4

Top Product

Discover our top product TLR4 Primary Antibody
anti-Toll-Like Receptor 4 (TLR4) (AA 751-835) antibody
TLR4 适用: 人, 小鼠, 大鼠, Cow WB, FACS, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 4 (TLR4) (AA 49-247) antibody
TLR4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (HRP)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal HRP

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (FITC)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal FITC

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Alkaline Phosphatase (AP))
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Biotin)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PE)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal PE

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (APC)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal APC

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PerCP)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal PerCP

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Atto 488)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal Atto 488

应用备注
- WB (1:500)
- IHC (1:50)
- optimal dilutions for assays should be determined by the user.
说明

2 μg/ml of ABIN361724 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
Vercellotti, Dalmasso, Schaid, Nguyen, Chen, Ericson, Abdulla, Killeen, Lindorfer, Taylor, Belcher: "Critical role of C5a in sickle cell disease." in: American journal of hematology, Vol. 94, Issue 3, pp. 327-337, (2019) (PubMed).

Nakano, Furube, Morita, Wanaka, Nakashima, Miyata: "Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain." in: Journal of neuroimmunology, Vol. 278, pp. 144-58, (2015) (PubMed).

Takano, Ohkusa, Otani, Min, Kadoyama, Minami, Sano, Matsuyama: "Lipid A-activated inducible nitric oxide synthase expression via nuclear factor-κB in mouse choroid plexus cells." in: Immunology letters, Vol. 167, Issue 2, pp. 57-62, (2015) (PubMed).
抗原

TLR4 (Toll-Like Receptor 4 (TLR4))

别名

TLR4 (TLR4 产品)

别名

ARMD10 antibody, CD284 antibody, TLR-4 antibody, TOLL antibody, Lps antibody, Ly87 antibody, Ran/M1 antibody, Rasl2-8 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody, TLR4 antibody, Tlr4 antibody

背景

The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa (6).

基因ID

7099

NCBI登录号

NP_612564

UniProt

O00206

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins