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HSP90 抗体

Name: HSP90 抗体
Brand: antibodies-online
SKU: ABIN361718
Availability: OutOfStock

HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 unconjugated

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (ABIN361717 and ABIN361718). (HSP90 抗体)

IHC

Hsp90 (H9010) Western Blotting, heat shock Hela. (HSP90 抗体)

Investigating the status of the canonical TGF-β pathway and levels of Hsp90 in the genetically paired SW480 primary and SW620 secondary tumour-derived colon cancer cell lines. (HSP90 抗体)

HSP90 complexes in hPSCs and hPSC-derived mDA neurons. (HSP90 抗体)

IHC

11 images

(11 references)

产品编号 ABIN361718

发货至: 中国

Datasheet as PDF

抗原 See all HSP90 抗体

HSP90 (Heat Shock Protein 90 (HSP90))
适用
111

63

61

30

26

21

20

20

11

10

10

10

9

8

6

6

6

6

5

5

5

4

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
人
宿主
85

66

3

1
小鼠
Compatible Secondaries
克隆类型
88

67
单克隆
标记
58

12

11

10

10

10

9

9

8

8

4

4

2
This HSP90 antibody is un-conjugated
应用范围
136

89

89

67

46

44

30

20

14

10

2

2

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)

特异性

Detects 90 kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.

交叉反应

小鸡, 犬, 鱼, Hamster, 人, 小鼠, 兔, 大鼠, Shark

纯化方法

Protein G Purified

免疫原

Recombinant human HSP90beta

克隆位点

H9010

亚型

IgG2a
anti-Heat Shock Protein 90 (HSP90) (Lys284), (Lys292) antibody
HSP90 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock Protein 90 (HSP90) (acLys284), (acLys292) antibody
HSP90 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock Protein 90 (HSP90) antibody
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock Protein 90 (HSP90) antibody (HRP)
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 HRP

anti-Heat Shock Protein 90 (HSP90) antibody (FITC)
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 FITC

anti-Heat Shock Protein 90 (HSP90) antibody (Biotin)
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 Biotin

anti-Heat Shock Protein 90 (HSP90) antibody (PE)
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 PE

anti-Heat Shock Protein 90 (HSP90) antibody (APC)
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 APC

anti-Heat Shock Protein 90 (HSP90) antibody (PerCP)
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 PerCP

anti-Heat Shock Protein 90 (HSP90) antibody (Atto 488)
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 Atto 488

应用备注
- WB (1:2500)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
说明

1 μg/ml of ABIN361717 was sufficient for detection of HSP90beta in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
de la Mare, Jurgens, Edkins: "Extracellular Hsp90 and TGFβ regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model." in: BMC cancer, Vol. 17, Issue 1, pp. 202, (2018) (PubMed).

Rodina, Wang, Yan, Gomes, Dunphy, Pillarsetty, Koren, Gerecitano, Taldone, Zong, Caldas-Lopes, Alpaugh, Corben, Riolo, Beattie, Pressl, Peter, Xu, Trondl, Patel, Shimizu, Bolaender, Yang, Panchal et al.: "The epichaperome is an integrated chaperome network that facilitates tumour survival. ..." in: Nature, Vol. 538, Issue 7625, pp. 397-401, (2017) (PubMed).

Jayaprakash, Dong, Zou, Bhatia, OBrien, Chen, Woodley, Li: "Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing." in: Journal of cell science, Vol. 128, Issue 8, pp. 1475-80, (2015) (PubMed).

Teigen, Orczewska, McLaughlin, OBrien: "Cold acclimation increases levels of some heat shock protein and sirtuin isoforms in threespine stickleback." in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol. 188, pp. 139-47, (2015) (PubMed).

Hunter, OHagan, Kenyon, Dhanani, Prinsloo, Edkins: "Hsp90 binds directly to fibronectin (FN) and inhibition reduces the extracellular fibronectin matrix in breast cancer cells." in: PLoS ONE, Vol. 9, Issue 1, pp. e86842, (2014) (PubMed).

Bessemer, Butler, Tunnah, Callaghan, Rundle, Currie, Dieni, MacCormack: "Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii." in: Nanotoxicology, pp. 1-10, (2014) (PubMed).

Patel, Yan, Seidler, Patel, Sun, Yang, Que, Taldone, Finotti, Stephani, Gewirth, Chiosis: "Paralog-selective Hsp90 inhibitors define tumor-specific regulation of HER2." in: Nature chemical biology, Vol. 9, Issue 11, pp. 677-84, (2013) (PubMed).

Espallergues, Teegarden, Veerakumar, Boulden, Challis, Jochems, Chan, Petersen, Deneris, Matthias, Hahn, Lucki, Beck, Berton: "HDAC6 regulates glucocorticoid receptor signaling in serotonin pathways with critical impact on stress resilience." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 13, pp. 4400-16, (2012) (PubMed).

Taldone, Zatorska, Patel, Zong, Rodina, Ahn, Moulick, Guzman, Chiosis: "Design, synthesis, and evaluation of small molecule Hsp90 probes." in: Bioorganic & medicinal chemistry, Vol. 19, Issue 8, pp. 2603-14, (2011) (PubMed).

Chen, Prior, Dargusch, Roberts, Riek, Eichmann, Chiruta, Akaishi, Abe, Maher, Schubert: "A novel neurotrophic drug for cognitive enhancement and Alzheimer's disease." in: PLoS ONE, Vol. 6, Issue 12, pp. e27865, (2011) (PubMed).

Gershburg, Murphy, Marschall, Gershburg: "Key motifs in EBV (Epstein-Barr virus)-encoded protein kinase for phosphorylation activity and nuclear localization." in: The Biochemical journal, Vol. 431, Issue 2, pp. 227-35, (2010) (PubMed).
抗原

HSP90 (Heat Shock Protein 90 (HSP90))

别名

HSP90 (HSP90 产品)

别名

EL52 antibody, HSP86 antibody, HSP89A antibody, HSP90A antibody, HSP90N antibody, HSPC1 antibody, HSPCA antibody, HSPCAL1 antibody, HSPCAL4 antibody, HSPN antibody, Hsp89 antibody, Hsp90 antibody, LAP2 antibody, git10 antibody, swo1 antibody, HSP90 antibody, htpG antibody, SCBAC25F8.08 antibody, 23.m06066 antibody, 17.m07646 antibody, HSP90-1 antibody, 143198_at antibody, 83 antibody, 83K HSP antibody, DMHSP82 antibody, E(sev)3A antibody, E(sina)2 antibody, HSP82 antibody, HSP83 antibody, ORF1 antibody, Su(Raf)3A antibody, anon-EST:Liang-2.53 antibody, anon-WO0068693 antibody, anon-WO0140519.209 antibody, clone 2.53 antibody, en(lz)3C/4C antibody, hsp84 antibody, l(3)j5C2 antibody, ms(3)08445 antibody, stc antibody, DmelCG1242 antibody, CG1242 antibody, Hsp86 antibody, Hspca antibody, 86kDa antibody, 89kDa antibody, AL024080 antibody, AL024147 antibody, Hsp86-1 antibody, hsp4 antibody, hsp86 antibody, hsp89 antibody, hsp90 antibody, hsp90a antibody, hspc1 antibody, hspca antibody, hspn antibody, lap2 antibody, Hsp90alpha antibody, heat shock protein 90 alpha family class A member 1 antibody, heat shock protein Hsp90 antibody, Hsp90 chaperone antibody, Heat shock protein 90 antibody, heat shock protein 90 antibody, chaperone protein HtpG antibody, molecular chaperone HtpG antibody, Heat Shock Protein 90 antibody, uncharacterized LOC100384473 antibody, Heat shock protein 83 antibody, heat shock protein 90, alpha (cytosolic), class A member 1 antibody, heat shock protein 90 alpha family class B member 1 antibody, heat shock protein 90kDa alpha family class A member 1 L homeolog antibody, heat shock protein HSP 90-alpha antibody, HSP90AA1 antibody, hsp90 antibody, HSP90 antibody, daf-21 antibody, htpG antibody, SCO7516 antibody, MAP_RS10510 antibody, TP04_0646 antibody, TP01_0934 antibody, APH_RS03525 antibody, GbCGDNIH1_0315 antibody, MAV_2118 antibody, HSP90C antibody, BBOV_IV008400 antibody, BBOV_III007380 antibody, ACICU_00312 antibody, ECL_01244 antibody, YE105_C1172 antibody, pco153543(105) antibody, Hsp83 antibody, Hsp90aa1 antibody, HSP90AB1 antibody, hsp90aa1.1.L antibody, LOC108698781 antibody

背景

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85 % sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species, having 60 % and 78 % amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.

基因ID

3326

NCBI登录号

NP_031381

UniProt

P08238

途径

M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals