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HSP70 抗体

Name: HSP70 抗体
Brand: antibodies-online
SKU: ABIN361708
Availability: OutOfStock

HSP70 适用: 人 WB, ELISA, IHC, IF, FACS, ICC, IEM, AA, BI 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated

Western blot analyses of the Hsp72 protein expression normalized to actin contents in the diaphragm in control animals (individual rats: young n = 4 and old emphn = 2) compared with the age-matched animals exposed to CMV for 5 days with (individual rats: young n = 3 and old n = 2) and without BGP-15 (individual rats: young n = 3 and old n = 3). (HSP70 抗体)

Western blot analysis of heat shock protein expression in fasted mouse livers. (HSP70 抗体)

Neutralization effect of an anti-heat shock protein 70 monoclonal antibody on the natural killer cell phenotype. (HSP70 抗体)

FACS

LRET assays of the interactions of TPR mutants of HOP with HSP70 and HSP90. (HSP70 抗体)

PEITC is a robust inducer of the heat shock response. (HSP70 抗体)

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708). (HSP70 抗体)

IHC

Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708). (HSP70 抗体)

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (ABIN361707 and ABIN361708). (HSP70 抗体)

ICC

IHC

11 images

(37 references)

产品编号 ABIN361708

发货至: 中国

Datasheet as PDF

抗原 See all HSP70 抗体

HSP70 (Heat Shock Protein 70 (HSP70))
适用
310

191

159

63

59

53

52

52

33

31

29

29

28

23

20

18

15

13

12

10

10

9

9

9

9

7

6

5

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
人
宿主
197

138

13

11

3
小鼠
Compatible Secondaries
克隆类型
211

150
单克隆
标记
105

29

26

23

21

19

18

15

14

14

9

5

5

5

5

5

5

5

5

4

4

4

3

3

2

1

1

1

1

1

1

1

1

1
This HSP70 antibody is un-conjugated
应用范围
340

142

133

108

108

105

91

66

62

61

39

28

22

20

20

6

4

2

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM), Antibody Array (AA), BioImaging (BI)

特异性

Detects ~70 kDa. Does not cross-react with HSC70 (HSP73).

交叉反应

C. elegans, Carp, 小鸡, Cow, 犬, Drosophila melanogaster, 豚鼠, Hamster, 人, 猴, 小鼠, Pig, 兔, 大鼠, 绵羊

纯化方法

Protein G Purified

免疫原

Human HSP70

克隆位点

C92F3A-5

亚型

IgG1
anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock Protein 70 (HSP70) antibody (HRP)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal HRP

anti-Heat Shock Protein 70 (HSP70) antibody (FITC)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal FITC

anti-Heat Shock Protein 70 (HSP70) antibody (Biotin)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal Biotin

anti-Heat Shock Protein 70 (HSP70) antibody (PE)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal PE

anti-Heat Shock Protein 70 (HSP70) antibody (APC)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal APC

anti-Heat Shock Protein 70 (HSP70) antibody (PerCP)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal PerCP

anti-Heat Shock Protein 70 (HSP70) antibody (Atto 488)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal Atto 488

anti-Heat Shock Protein 70 (HSP70) antibody (Atto 594)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal Atto 594

anti-Heat Shock Protein 70 (HSP70) antibody (Atto 390)
HSP70 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 兔 Polyclonal Atto 390

应用备注
- WB (1:1000)
- IHC (1:10000)
- ICC/IF (1:1000)
- FACS (1:1000)
- optimal dilutions for assays should be determined by the user.
说明

1 μg/ml of ABIN361707 was sufficient for detection of HSP70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
Kuta, Larochelle, Fernandez, Pal, Minotti, Tibshirani, St Louis, Gentil, Nalbantoglu, Hermann, Durham et al.: "Depending on the stress, histone deacetylase inhibitors act as heat shock protein co-inducers in motor neurons and potentiate arimoclomol, exerting neuroprotection through multiple mechanisms in ALS ..." in: Cell stress & chaperones, Vol. 25, Issue 1, pp. 173-191, (2020) (PubMed).

Bhattacharya, Bernasconi, Picard: "Luminescence resonance energy transfer between genetically encoded donor and acceptor for protein-protein interaction studies in the molecular chaperone HSP70/HSP90 complexes." in: Scientific reports, Vol. 8, Issue 1, pp. 2801, (2019) (PubMed).

Qiao, Jin, Pang, Moskophidis, Mivechi: "The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis." in: The Journal of cell biology, Vol. 216, Issue 3, pp. 723-741, (2017) (PubMed).

Dayalan Naidu, Sutherland, Zhang, Risco, de la Vega, Caunt, Hastie, Lamont, Torrente, Chowdhry, Benjamin, Keyse, Cuenda, Dinkova-Kostova: "Heat Shock Factor 1 Is a Substrate for p38 Mitogen-Activated Protein Kinases." in: Molecular and cellular biology, Vol. 36, Issue 18, pp. 2403-17, (2017) (PubMed).

Ogilvie, Cacciani, Akkad, Larsson: "Targeting Heat Shock Proteins Mitigates Ventilator Induced Diaphragm Muscle Dysfunction in an Age-Dependent Manner." in: Frontiers in physiology, Vol. 7, pp. 417, (2016) (PubMed).

Ishikawa, Sakurai: "Heat-induced expression of the immediate-early gene IER5 and its involvement in the proliferation of heat-shocked cells." in: The FEBS journal, Vol. 282, Issue 2, pp. 332-40, (2015) (PubMed).

Katoh, Kubota, Kita, Nakatsu, Aoki, Mori, Maenaka, Takeda, Kidokoro: "Heat shock protein 70 regulates degradation of the mumps virus phosphoprotein via the ubiquitin-proteasome pathway." in: Journal of virology, Vol. 89, Issue 6, pp. 3188-99, (2015) (PubMed).

Chittoor-Vinod, Lee, Judge, Notterpek: "Inducible HSP70 is critical in preventing the aggregation and enhancing the processing of PMP22." in: ASN neuro, Vol. 7, Issue 1, (2015) (PubMed).

Sheppard, Sun, Khammash, Giffard: "Overexpression of heat shock protein 72 attenuates NF-?B activation using a combination of regulatory mechanisms in microglia." in: PLoS computational biology, Vol. 10, Issue 2, pp. e1003471, (2014) (PubMed).

Wijeratne, Xu, Scherz-Shouval, Marron, Rocha, Liu, Costa-Lotufo, Santagata, Lindquist, Whitesell, Gunatilaka: "Structure-activity relationships for withanolides as inducers of the cellular heat-shock response." in: Journal of medicinal chemistry, Vol. 57, Issue 7, pp. 2851-63, (2014) (PubMed).

Akkad, Corpeno, Larsson: "Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model." in: PLoS ONE, Vol. 9, Issue 4, pp. e92622, (2014) (PubMed).

Eroglu, Kimbler, Pang, Choi, Moskophidis, Yanasak, Dhandapani, Mivechi: "Therapeutic inducers of the HSP70/HSP110 protect mice against traumatic brain injury." in: Journal of neurochemistry, Vol. 130, Issue 5, pp. 626-41, (2014) (PubMed).

Muralidharan, Ambade, Fulham, Deshpande, Catalano, Mandrekar: "Moderate alcohol induces stress proteins HSF1 and hsp70 and inhibits proinflammatory cytokines resulting in endotoxin tolerance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 4, pp. 1975-87, (2014) (PubMed).

Kanegasaki, Matsushima, Shiraishi, Nakagawa, Tsuchiya: "Macrophage inflammatory protein derivative ECI301 enhances the alarmin-associated abscopal benefits of tumor radiotherapy." in: Cancer research, Vol. 74, Issue 18, pp. 5070-8, (2014) (PubMed).

Berger, Ivanova, Gareau, Scherrer, Mazroui, Strub: "Direct binding of the Alu binding protein dimer SRP9/14 to 40S ribosomal subunits promotes stress granule formation and is regulated by Alu RNA." in: Nucleic acids research, Vol. 42, Issue 17, pp. 11203-17, (2014) (PubMed).

Dang, Tanabe, Tanaka, Tokumoto, Misumi, Saeki, Fujikuni, Ohdan: "Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation." in: PLoS ONE, Vol. 9, Issue 10, pp. e110748, (2014) (PubMed).

Engels, Bilgic, Pinto, Vasquez, Wollschläger, Steinbrenner, Kellermann, Akhyari, Lichtenberg, Boeken: "A cardiopulmonary bypass with deep hypothermic circulatory arrest rat model for the investigation of the systemic inflammation response and induced organ damage." in: Journal of inflammation (London, England), Vol. 11, pp. 26, (2014) (PubMed).

Richter, Viergutz, Schwerin, Weitzel: "Prostaglandin E synthase interacts with inducible heat shock protein 70 after heat stress in bovine primary dermal fibroblast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 87, Issue 1, pp. 61-7, (2014) (PubMed).

Seguin, Morelli, Vinet, Amore, De Biasi, Poletti, Rubinsztein, Carra: "Inhibition of autophagy, lysosome and VCP function impairs stress granule assembly." in: Cell death and differentiation, Vol. 21, Issue 12, pp. 1838-51, (2014) (PubMed).

Bauckman, Haller, Flores, Nanjundan: "Iron modulates cell survival in a Ras- and MAPK-dependent manner in ovarian cells." in: Cell death & disease, Vol. 4, pp. e592, (2013) (PubMed).

show more references
抗原

HSP70 (Heat Shock Protein 70 (HSP70))

别名

HSP70 (HSP70 产品)

别名

APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody

背景

HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.

基因ID

3303

NCBI登录号

NP_005336

UniProt

P0DMV8, P0DMV9