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HSP70/HSC70 抗体

Name: HSP70/HSC70 抗体
Brand: antibodies-online
SKU: ABIN361706
Availability: OutOfStock

HSC70-4 适用: 人 WB, IP, IHC, FACS, IEM, ICC, IF 宿主: 小鼠 Monoclonal N27F3-4 unconjugated

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27F3-4 (ABIN361705 and ABIN361706). (HSP70/HSC70 抗体)

IHC

Western Blot analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27F3-4 (ABIN361705 and ABIN361706). (HSP70/HSC70 抗体)

Hsp70 (N27), cell lines. (HSP70/HSC70 抗体)

Hsc70/Hsp70 suppresses levels of mature CFTR. (HSP70/HSC70 抗体)

5 images

(8 references)

产品编号 ABIN361706

发货至: 中国

Datasheet as PDF

抗原 See all HSP70/HSC70 (HSC70-4) products

HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))
适用
35

33

25

23

23

23

23

22

22

21

21

21

20

20

20

16

12

11

10

10

10

4

3

1

1

1

1

1

1

1
人
宿主
48

2
小鼠
Compatible Secondaries
克隆类型
48

2
单克隆
标记
11

5

5

5

4

4

4

4

4

4
This HSP70/HSC70 antibody is un-conjugated
应用范围
50

42

41

21

19

19

19

10

5

2
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF)

特异性

Detects ~72 (HSP) and ~73 kDa (HSC).

交叉反应

Beluga, C. elegans, 小鸡, Cow, 犬, Drosophila melanogaster, 鱼, 豚鼠, Hamster, 人, 猴, 小鼠, Pig, 植物, 兔, 大鼠, 绵羊, 非洲爪蟾

纯化方法

Protein G Purified

免疫原

Recombinant HSP70/HSC70

克隆位点

N27F3-4

亚型

IgG1
anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 unconjugated

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (HRP)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 HRP

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (FITC)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 FITC

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (Biotin)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 Biotin

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (PE)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 PE

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (APC)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 APC

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (PerCP)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 PerCP

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (Atto 488)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 Atto 488

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (Atto 594)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 Atto 594

anti-Heat Shock Protein Cognate 4 (HSC70-4) antibody (Atto 390)
HSC70-4 适用: 小鸡 WB, IP, IHC, ICC, IF 宿主: 小鼠 Monoclonal BB70 Atto 390

应用备注
- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:50)
- optimal dilutions for assays should be determined by the user.
说明

1 μg/ml of ABIN361705 was sufficient for detection of HSP70/HSC70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
Wang, Kojima, Mobley, West: "Proteomic analysis of urinary extracellular vesicles reveal biomarkers for neurologic disease." in: EBioMedicine, Vol. 45, pp. 351-361, (2019) (PubMed).

Preusse-Prange, Modrow, Schwark, von Wurmb-Schwark: "Detection of constitutive and inducible HSP70 proteins in formalin fixed human brain tissue." in: Forensic science international, Vol. 235, pp. 62-7, (2014) (PubMed).

Morshed, Ma, Latif, Davies: "How one TSH receptor antibody induces thyrocyte proliferation while another induces apoptosis." in: Journal of autoimmunity, Vol. 47, pp. 17-24, (2013) (PubMed).

Sun, Prince, Manjarrez, Scroggins, Matts: "Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins." in: Biochimica et biophysica acta, Vol. 1823, Issue 6, pp. 1092-101, (2012) (PubMed).

Modrow, Preusse-Prange, Meyer, Harder, Schwark, von Wurmb-Schwark: "Highly reliable quantification of proteins such as members of the HSP70 superfamily based on the grey scale index via immune detection stained bands on a Western blot." in: Forensic science international, Vol. 222, Issue 1-3, pp. 256-8, (2012) (PubMed).

Mutsvunguma, Moetlhoa, Edkins, Luke, Blatch, Knox: "Theiler's murine encephalomyelitis virus infection induces a redistribution of heat shock proteins 70 and 90 in BHK-21 cells, and is inhibited by novobiocin and geldanamycin." in: Cell stress & chaperones, Vol. 16, Issue 5, pp. 505-15, (2011) (PubMed).

Chen, Prior, Dargusch, Roberts, Riek, Eichmann, Chiruta, Akaishi, Abe, Maher, Schubert: "A novel neurotrophic drug for cognitive enhancement and Alzheimer's disease." in: PLoS ONE, Vol. 6, Issue 12, pp. e27865, (2011) (PubMed).

Olkku, Leskinen, Lammi, Hynynen, Mahonen: "Ultrasound-induced activation of Wnt signaling in human MG-63 osteoblastic cells." in: Bone, Vol. 47, Issue 2, pp. 320-30, (2010) (PubMed).
抗原

HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))

别名

HSP70/HSC70 (HSC70-4 产品)

别名

BAP74 antibody, CG4264 antibody, Dmel\\CG4264 antibody, E(csp)1545 antibody, E(nd)195 antibody, HSC-70 antibody, HSC4 antibody, HSC70 antibody, HSC70-4 antibody, Hsc-4 antibody, Hsc4 antibody, Hsc4p antibody, Hsc70 antibody, Hsp-c4 antibody, Hsp70 antibody, anon-WO0118547.237 antibody, bs17d06.y1 antibody, dhsc70 antibody, hsc4 antibody, hsc70 antibody, hsc70-4 antibody, hsp70 antibody, i190 antibody, l(3)03550 antibody, l(3)L3929 antibody, l(3)j7A4 antibody, scd antibody, Heat shock protein cognate 4 antibody, Hsc70-4 antibody

背景

HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.

基因ID

3303

NCBI登录号

NP_005336

UniProt

P0DMV8, P0DMV9