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DNAJB1 抗体

DNAJB1 适用: Saccharomyces cerevisiae WB, ELISA, IP 宿主: 小鼠 Monoclonal 2A7-H6 unconjugated
产品编号 ABIN361662
发货至: 中国
  • 抗原 See all DNAJB1 抗体
    DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
    适用
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    Saccharomyces cerevisiae
    宿主
    • 66
    • 37
    小鼠
    克隆类型
    • 66
    • 37
    单克隆
    标记
    • 51
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    This DNAJB1 antibody is un-conjugated
    应用范围
    • 88
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    特异性
    Detects ~40 kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.
    交叉反应
    Saccharomyces cerevisiae
    纯化方法
    Protein G Purified
    免疫原
    Full length protein HSP40 (YDJ1)
    克隆位点
    2A7-H6
    亚型
    IgG1 kappa
    Top Product
    Discover our top product DNAJB1 Primary Antibody
  • 应用备注
    • WB (1:2000)
    • optimal dilutions for assays should be determined by the user.
    说明

    0.5 μg/ml of ABIN361661 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    -20°C
  • Knighton, Nitika, Waller, Strom, Wolfgeher, Reitzel, Truman: "Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock." in: Journal of proteomics, Vol. 206, pp. 103416, (2020) (PubMed).

    Sluder, Nitika, Knighton, Truman: "The Hsp70 co-chaperone Ydj1/HDJ2 regulates ribonucleotide reductase activity." in: PLoS genetics, Vol. 14, Issue 11, pp. e1007462, (2019) (PubMed).

  • 抗原
    DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
    别名
    HSP40 (DNAJB1 产品)
    别名
    HSPF1 antibody, Hdj1 antibody, Hsp40 antibody, RSPH16B antibody, Sis1 antibody, CG10578 antibody, DNAJ-1 antibody, DNAJ1 antibody, DROJ1 antibody, Dmel\\CG10578 antibody, DnaJ1 64EF antibody, DroJ1 antibody, EU3500 antibody, HSP40 antibody, HSP40/HDJ1 antibody, Hsp-40 antibody, anon-WO0140519.166 antibody, anon-WO0172774.135 antibody, dHDJ1 antibody, dHDJ1/HSP40 antibody, dHdj1 antibody, dhdJ1 antibody, dhdj-1 antibody, dhdj1 antibody, dnaJ-1 antibody, droj1 antibody, hsp40 antibody, dnajb4 antibody, hdj1 antibody, hspf1 antibody, sis1 antibody, DnaJ-5 antibody, MAS5 antibody, dnaJ antibody, 0610007I11Rik antibody, dnajb1 antibody, zf-Hsp40 antibody, zgc:101068 antibody, DnaJ heat shock protein family (Hsp40) member B1 antibody, DnaJ-like-1 antibody, DnaJ (Hsp40) homolog 5 antibody, type I HSP40 co-chaperone YDJ1 antibody, DnaJ protein antibody, molecular chaperone DnaJ antibody, DnaJ (Hsp40) homolog, subfamily B, member 1a antibody, DNAJB1 antibody, Dnajb1 antibody, DnaJ-1 antibody, dnajb1 antibody, DnaJ-5 antibody, YDJ1 antibody, dnaJ antibody, dnajb1a antibody
    背景
    Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
    基因ID
    855661
    NCBI登录号
    NP_014335
    UniProt
    P25491
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