Specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204 in Western Blots of UV treated Jurkat Cells or in a rat brain lysate. Immunolabeling is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Human, Mouse, Rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody.
交叉反应
人, 小鼠, 大鼠
预测反应
bovine, canine, chicken, mouse, non-human primates, Xenopus, zebra fish
纯化方法
Antigen Affinity Purified from Pooled Serum
免疫原
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH