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EPH Receptor B2 抗体 (pTyr317)

EPHB2 适用: 小鸡 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN361467
发货至: 中国
  • 抗原 See all EPH Receptor B2 (EPHB2) 抗体
    EPH Receptor B2 (EPHB2)
    抗原表位
    • 8
    • 8
    • 6
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    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr317
    适用
    • 59
    • 29
    • 13
    • 5
    • 2
    • 1
    • 1
    小鸡
    宿主
    • 46
    • 15
    • 5
    • 2
    克隆类型
    • 48
    • 20
    多克隆
    标记
    • 39
    • 6
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EPH Receptor B2 antibody is un-conjugated
    应用范围
    • 47
    • 46
    • 25
    • 14
    • 11
    • 10
    • 9
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    特异性
    Specific for the ~46k EphrinB protein phosphorylated at Tyr317. The immunolabeling of the EphrinB band is blocked by treatment with (-phosphatase.
    交叉反应
    小鼠, 大鼠
    预测反应
    bovine, chicken, human, mouse, Xenopus, zebra fish
    纯化方法
    Antigen Affinity Purified from Pooled Serum
    免疫原
    Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 conjugated to KLH
    Top Product
    Discover our top product EPHB2 Primary Antibody
  • 应用备注
    Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
    储存条件
    -20 °C
  • 抗原
    EPH Receptor B2 (EPHB2)
    别名
    EPHB2 (EPHB2 产品)
    背景
    EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001). Anti-Phospho-Tyr317 EphrinB Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr317 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphrinB is completely eliminated by treatment with (-Ptase.
    分子量
    '46 kDa
    基因ID
    396513
    UniProt
    P28693
    途径
    RTK signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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