Tryptophan Hydroxylase 1 抗体
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- 抗原 See all Tryptophan Hydroxylase 1 (TPH1) 抗体
- Tryptophan Hydroxylase 1 (TPH1)
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适用
- 兔
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This Tryptophan Hydroxylase 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Specific for the ~55k tryptophan hydroxylase protein.
- 交叉反应
- 人, 大鼠
- 预测反应
- wide variety mammalian
- 纯化方法
- Antigen Affinity Purified from Pooled Serum
- 免疫原
- Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE
- Top Product
- Discover our top product TPH1 Primary Antibody
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- 应用备注
- Recommended Dilution: WB: 1:1000 IHC (frozen sections, Haycock et al., 2002): 1:1000 Quality Control: Western blots performed on each lot.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
- 储存条件
- -20 °C
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- 抗原
- Tryptophan Hydroxylase 1 (TPH1)
- 别名
- TPH1 (TPH1 产品)
- 背景
- Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001). Anti-Tryptophan Hydroxylase Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.
- 分子量
- '55 kDa
- 基因ID
- 100101558
- UniProt
- P17290
- 途径
- Feeding Behaviour
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