Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
Specific for the ~29 kDa Calretinin protein in Western blots. The antibody also works well for immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections. It is expected that this antibody will also react with human, mouse , non-human primates.
Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000). Anti-Calretinin Western blot of rat cerebellum (CB) lysate showing specific immunolabeling of the ~ 29k calretinin protein.