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NEFH 抗体

This anti-NEFH antibody is a 小鼠 单克隆 antibody detecting NEFH in WB 和 IF. Suitable for Cow.
产品编号 ABIN361350
发货至: 中国

Quick Overview for NEFH 抗体 (ABIN361350)

抗原

See all NEFH 抗体
NEFH (Neurofilament, Heavy Polypeptide (NEFH))

适用

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Cow

宿主

  • 80
  • 63
  • 9
小鼠

克隆类型

  • 98
  • 54
单克隆

标记

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This NEFH antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)

克隆位点

NAP4
  • 特异性

    Specific for the ~200k Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

    交叉反应

    小鸡, 哺乳动物

    纯化方法

    Protein G purified culture supernatant

    免疫原

    Purified bovine NF-H

    亚型

    IgG1
  • 应用备注

    Recommended Dilution: WB: 1:5,000 IF: 1:500 Quality Control: Western blots performed on each lot.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    total IgG fraction + 10 mM Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C
  • 抗原

    NEFH (Neurofilament, Heavy Polypeptide (NEFH))

    别名

    NEFH

    背景

    Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). Anti-Neurofilament H Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein.

    分子量

    '200 kDa

    基因ID

    4744

    UniProt

    P12036
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