NEFH 抗体
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- 抗原 See all NEFH 抗体
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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适用
- Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NEFH antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Specific for the ~200k Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
- 交叉反应
- 小鸡, 哺乳动物
- 纯化方法
- Protein G purified culture supernatant
- 免疫原
- Purified bovine NF-H
- 克隆位点
- NAP4
- 亚型
- IgG1
- Top Product
- Discover our top product NEFH Primary Antibody
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- 应用备注
- Recommended Dilution: WB: 1:5,000 IF: 1:500 Quality Control: Western blots performed on each lot.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- total IgG fraction + 10 mM Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
- 别名
- NEFH (NEFH 产品)
- 背景
- Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). Anti-Neurofilament H Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein.
- 分子量
- '200 kDa
- 基因ID
- 4744
- UniProt
- P12036
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