Protein A column, followed by peptide affinity purification.
免疫原
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y248 of human PHB2.
ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
0.25 mg/mL
缓冲液
PBS with 0.09 % (W/V) sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.Synonyms: B-cell receptor-associated protein BAP37, BAP, BAP37, D-prohibitin, PHB2, REA, Repressor of estrogen receptor activity
分子量
33165 Da predicted from amino acid sequence. Theoretical pI: 9.83.