Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
免疫原
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region (between 308-337aa) of human NAGK.
N-acetylglucosamine kinase (NAGK) converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent salvage enzyme of amino sugar metabolism in mammals. The predicted 344-amino acid NAGK protein contains the 5 sequence motifs necessary for the binding of ATP by sugar kinases. NAGK shares 91.6 % amino acid similarity with mouse Nagk, for which enzyme activity is detectable in all mouse tissues examined, with highest enzymatic activity in testis. It is hypothesized that NAGK has a general role in the catabolic pathways of GlcNAc as well as of ManNAc.Synonyms: N-acetyl-D-glucosamine kinase