Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
免疫原
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN.
ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
0.25 mg/mL
缓冲液
PBS with 0.09 % (W/V) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
抗原
Separase (ESPL1)
(Extra Spindle Poles Like 1 (ESPL1))
ESP1 antibody, SEPA antibody, mp:zf637-3-001691 antibody, AL024103 antibody, AU045071 antibody, Cerp antibody, PRCE antibody, SSE antibody, extra spindle pole bodies like 1, separase antibody, extra spindle poles like 1 antibody, extra spindle pole bodies 1, separase antibody, ESPL1 antibody, espl1 antibody, Espl1 antibody
背景
The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securinsSynonyms: Caspase-like protein ESPL1, ESP1, Extra spindle poles-like 1 protein, KIAA0165, Separase