This antibody detects IKKb pTyr199. Predicted to cross react with Mouse (100 % Antigen Homology).
纯化方法
Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
免疫原
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.
ELISA: 1/1,000. Western Blot: 1/100-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
0.25 mg/mL
缓冲液
PBS with 0.09 % (W/V) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
抗原
IKBKB
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.Synonyms: I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta