ube3a 抗体 (C-Term)
Quick Overview for ube3a 抗体 (C-Term) (ABIN357571)
抗原
See all ube3a 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody is specific to UBE3A (C-term).
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纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE3A.
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亚型
- Ig Fraction
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anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 51-150) antibody
ube3a 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) antibodyube3a 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 836-865), (C-Term) antibodyube3a 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB4440 unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) antibodyube3a 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 300-590) antibodyube3a 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (Middle Region) antibodyube3a 适用: 人, 小鼠, 大鼠, 兔, 犬, 豚鼠, 马, 斑马鱼, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 83-234) antibodyube3a 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) antibodyube3a 适用: 人 IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) antibodyube3a 适用: 人 WB, IHC, FACS, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 158-407) antibodyube3a 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- ube3a (Ubiquitin Protein Ligase E3A (ube3a))
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别名
- UBE3A
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背景
- UBE3A interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein. UBE3A binds UBQLN1 and UBQLN2. Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830], also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove. UBE3A contains 1 HECT-type E3 ubiquitin-protein ligase domain.Synonyms: E6AP, EPVE6AP, HPVE6A, Human papillomavirus E6-associated protein, NY-REN-54, Oncogenic protein-associated protein E6-AP, Ubiquitin-protein ligase E3A
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分子量
- 100645 Da
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基因ID
- 7337, 9606
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UniProt
- Q05086
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
抗原
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