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UBE2I 抗体 (N-Term)

UBE2I 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN356594
发货至: 中国
  • 抗原 See all UBE2I 抗体
    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
    抗原表位
    • 6
    • 5
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    N-Term
    适用
    • 73
    • 33
    • 32
    • 8
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    宿主
    • 62
    • 10
    • 2
    克隆类型
    • 52
    • 22
    多克隆
    标记
    • 36
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    This UBE2I antibody is un-conjugated
    应用范围
    • 50
    • 30
    • 22
    • 9
    • 7
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    特异性
    This antibody is specific to UBE2I (N-term). Predicted to cross react with Mouse and DANRE (100 % Antigen Homology).
    纯化方法
    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
    免疫原
    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2I.
    亚型
    Ig Fraction
    Top Product
    Discover our top product UBE2I Primary Antibody
  • 应用备注
    ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.25 mg/mL
    缓冲液
    PBS containing 0.09 % (W/V) Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原
    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
    别名
    UBC9 / UBE2I (UBE2I 产品)
    背景
    UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBalpha, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I
    基因ID
    7329, 9606
    UniProt
    P63279
    途径
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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