Flow Cytometry: Identification and enumeration of CD5+ cells. (Ref.15)Immunoprecipitation. (Ref.15)Immunohistochemistry on Frozen Sections. (Ref.15)T Cell Activation. (Ref.15)Western blotting. (Ref.15) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (Ref.1-3) It has been identified as the major ligand of the B-cell antigen CD72. (Ref.4,5) The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in Ref.6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (Ref.7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (Ref.13,14)Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5