Immunohistochemistry: 1/50-1/100 (30-60 min at RT). (See Ref.2)Immunofluorescence. (See Ref.2)Western blotting: < / = 1 μg/mL (See Ref.2)Electron Microscopic Immunolocalization. (See Ref.2) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.2 mg/mL
缓冲液
100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Shelf life: One year from despatch.
The fibrillins are extracellular matrix glycoproteins which occur as a major component of a subset of connective tissue microfibrils. These microfibrils have a beaded appearance and a cross-sectional diameter of 10-12 nm. In elastic tissues, these structures are thought to provide the scaffold onto which elastin is assembled to form elastic fibers, although their function in non-elastic tissues is unclear.(Ref.1) In Immunohistochemical staining of paraformaldehyde-fixed human skin, mAb 11C1.3 labels the elastic framework and more specifically the oxytalantic network close to the epidermis. The antibody also exhibits discrete staining of normal fibroblasts in culture and of extracellular (elastin) fibrils in a variety of tissues.(Ref.2,3) There is an aberrant expression of fibrillin in Marfan syndrome.(Ref.4)Synonyms: FBN, FBN1