Nestin 抗体 (AA 1464-1614)
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- 抗原 See all Nestin (NES) 抗体
- Nestin (NES)
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抗原表位
- AA 1464-1614
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nestin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- IHC-plus™
- 特异性
- This antibody is specific for human nestin. Species cross-reactivity: This antibody recognizes human nestin protein. It does not cross-react with rodent protein.
- 无交叉反应
- Rodents
- 纯化方法
- Protein G purified
- 免疫原
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A fragment of the human nestin protein corresponding to residues 1464-1614.
Type of Immunogen: Protein - 亚型
- IgG1 kappa
- Top Product
- Discover our top product NES Primary Antibody
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anti-Nestin (NES) (AA 178-399) antibody
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anti-Nestin (NES) antibodyNES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated
anti-Nestin (NES) (AA 698-844) antibodyNES 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal NES-2911 unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 681-750) antibodyNES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ICC, IF, IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1:1000 - 1:5000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000. Doublet band seen ~220-240kD. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short term: 4°C
Long term: -20°C.
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