A synthetic peptide from AA 100-144 of human SOD1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
IHC, WB (confirmed by recombinant protein). A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Function: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalytic activity: 2 superoxide + 2 H(+) = O(2) + H(2)O(2). Subcellular location: Cytoplasm. Involvement in disease: Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10 % of cases leading to familial forms. The protein (both wild-type and ALS1 variants) has a tendency to form fibrillar aggregates in the absence of the intramolecular disulfide bond or of bound zinc ions. These aggregates may have cytotoxic effects. Zinc binding promotes dimerization and stabilizes the native form.,Signal Transduction,SOD1, Superoxide dismutase 1, SODC