A synthetic peptide from AA 130-180 of human RhoF conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.
IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
有效期
12 months
抗原
RhoF (RHOF)
(Rho-related GTP-binding protein RhoF (RHOF))
ARHF antibody, RIF antibody, AI845056 antibody, AV026554 antibody, Arhf antibody, Ifld1 antibody, Rif antibody, ras homolog family member F, filopodia associated antibody, ras homolog family member F (in filopodia) antibody, RHOF antibody, Rhof antibody
背景
Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology. Subcellular location: Cell membrane, Lipid-anchor, Cytoplasmic sidePotential. Cytoplasm âÄ° cytoskeleton.,Rho Family,Rho family GTPase Rif, Rho in filopodia, ARHF, RIF, Rho in filopodia