LC3C (MAP1LC3C)
(Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))
适用
人
宿主
绵羊
克隆类型
多克隆
标记
This LC3C antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC)
特异性
Specific for MAP1LC3C.
交叉反应
人
交叉反应 (详细)
Other species not yet tested.
纯化方法
IgG
免疫原
A synthetic peptide from human microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C, MAP1A/MAP1B LC3 C) conjugated to an immunogenic carrier protein has been used as the immunogen.
IHC, WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
有效期
12 months
抗原
LC3C (MAP1LC3C)
(Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane, Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane, Lipid-anchor. Note: LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.,Mammalian Autophagy,Microtubule-associated proteins 1A/1B light chain 3C, MAP1LC3C, MAP1A/MAP1B LC3 C, MAP1A/1B light chain 3 C, MAP1 light chain 3-like protein 3, Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3C