A synthetic peptide from c-terminal of rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.
IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.,Neurodegeneration,38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase