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- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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适用
- Aequorea victoria
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This GFP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Specific for EGFP/GFP/Venus
- 免疫原
- Synthetic peptides (a pentamer) from EGFP/GFP/Venus conjugated to an immunogenic carrier protein was used as the antigen.
- 亚型
- IgG
- Top Product
- Discover our top product GFP Primary Antibody
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- 应用备注
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Use at a concentration of 10-50 µg/ml.
The optimal working concentration should be determined by the end user. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute in 100 µL of MQ water. Centrifuge to remove any insoluble material.
- 注意事项
- Avoid freeze and thaw cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- 有效期
- 12 months
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Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).
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Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- Abstract
- GFP 产品
- 别名
- green fluorescent protein antibody, gfp antibody
- 背景
- GFP is a naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin’s blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Also known as: Enhanced Green Fluorescence Protein.
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