ATG4C
(Autophagy related 4C Cysteine Peptidase (ATG4C))
适用
人
宿主
绵羊
克隆类型
多克隆
标记
This ATG4C antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC)
特异性
Specific for ATG4C.
交叉反应
人, 小鼠, 大鼠
交叉反应 (详细)
Other species not yet tested.
纯化方法
IgG
免疫原
A synthetic peptide from human cysteine protease ATG4C (autophagin-3, APG4C, AUTL1, AUTL3) conjugated to an immunogenic carrier protein has been used as the antigen.
IHC, WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
有效期
12 months
抗原
ATG4C
(Autophagy related 4C Cysteine Peptidase (ATG4C))
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, liver and testis.,Mammalian Autophagy,ATG4C, autophagin-3, APG4C, AUTL1, AUTL3, APG4-C, FLJ14867, ATG4 autophagy related 4 homolog C (S. cerevisiae), Autophagy-related protein 4 homolog C, Autophagy-related cysteine endopeptidase 3, AUT-like 3 cysteine endopeptidase